Mutations affectant le gène de structure de la 2-céto-3-désoxy-6-P-gluconate aldolase chez E. coli K 12

In order to determine the nature of KDPG-aldolase negative mutations (described in a recent paper) we have studied revertants to wild type. The structure of restored KDPG-aldolase in two revertants is very different with regard to wild type aldolase activity (modified thermosensibility, Km and VM). These restored aldolases like the wild type aldolase are under the control of the regulator gene (kdg R). The observation that one of these revertants maps in the eda locus demonstrates that this locus is the structural gene of KDPG-aldolase in E. coli K 12.