Population survey of CCR5 delta32, CCR5 m303, CCR2b 64I, and SDF1 3'A allele frequencies in indigenous Chinese healthy individuals, and in HIV-1-infected and HIV-1-uninfected individuals in HIV-1 risk groups.

The aim of this study is to determine in indigenous Chinese ethnic groups the frequencies of the chemokine (SDF1 3’A) and chemokine receptors (CCR5 -32 CCR5 m303 and CCR2b 64I) HIV-1/AIDS restriction alleles. The study includes two cohorts; the first comprised 3165 indigenous healthy subjects representing eight ethnic groups: Han (n = 1406) Uygur (n = 316) Mongolia (n = 134) Hui (n = 386) Tibetan (n = 330) Zhuang (n = 378) Dai (n = 101) and Jingbo (n = 114). The second cohort consisted of 330 HIV-1–infected (86 subjects infected by sexual transmission and 198 subjects infected by HIV-1–contaminated blood or by sharing injection equipment; the remaining 46 subjects said nothing about HIV-1 transmission) and 474 HIV-1–uninfected Han Chinese belonging to one of two HIV-1 high-risk groups: intravenous drug users (n=215) and individuals with sexually transmitted diseases (n = 259). Genotypes for the four genes were obtained using PCR (CCR5 -32) or PCR-restriction fragment length polymorphism. Randomly selected amplified PCR products were further confirmed by direct DNA sequencing. The variant allele frequencies were determined to be 0% to 3.48% for CCR5 -32 0% for CCR5 m303 16.23% to 28.79% for CCR2b 64I and 17.70% to 27.76% for SDF1 3’A in Chinese healthy individuals from eight ethnic groups. These findings show that allele frequencies differ among the eight Chinese ethnic groups for CCR5 -32 CCR2b 64I and SDF1 3’A and that the CCR5 m303 and CCR5 -32 mutant alleles were absent or infrequent in Chinese which may be helpful for studies of specific anti-HIV-1 vaccine trials and coreceptor inhibitor drug targets in Chinese populations. Furthermore we observed no significant differences in allele or genotypic frequencies between HIV-1–infected and HIV-1–uninfected groups from the Han ethnic group. Our finding is the first reporting that there is likely no effect of the examined polymorphisms in our study on HIV-1 transmission in the Chinese Han population However the genetic effects of these and other AIDS-modifying polymorphisms on the pathogenesis and clinical outcome of HIV-1/AIDS diseases is under investigation in Chinese populations. (authors)