Evaluation of Check-Points Check-MDR CT103 PCR-Microarray Kit for Detection and Classification of ESBL, AmpC and Carbapenemase Genes

revised) Multi-drug resistant Gram-negative bacilli (MDR-GNB) are of grave concern to physicians and laboratorians worldwide. The ability for clinical laboratories to rapidly identify MDR-GNB within their institutions remains a sentinel defense in containment and prevention of further dissemination. Tools for identifying MDR-GNB and their associated resistance mechanisms are largely focused on phenotypic methods such as antibiotic-selective culture methods, commercial chromogenic media, commercial antimicrobial susceptibility testing, or as part of a cascaded algorithm using minimum inhibitory concentration breakpoints, followed with by confirmatory specific tests. Polymerase chain reaction assays targeting specific β-lactamase and carbapenemase genes, are also available. MDR-GNBs often carry plasmids which harbor multiple genes mediating antimicrobial resistance. Many resistance mechanisms, especially those encoded on plasmids, can include other resistance genes Given these factors, a broad, multiplex approach to molecular genotyping are is optimal to comprehensively type MDR- GNB isolates.