Formalin-fixed paraffin-embedded (FFPE) sample nucleic acid library, construction method and FFPE sample analysis method

The present invention is in the field of molecular biology, specifically discloses an FFPE sample nucleic acid library, which method and analysis method of constructing FFPE samples. Specifically, the nucleic acid libraries of the invention a nucleic acid extraction through FFPE samples; fragmented nucleic acid; repair the DNA fragment ends and 3 'end of the connecting "A" bases; and quantitative connection joint; segment selection; like the PCR amplification step. FFPE library constructed in the present invention can capture a target sequence and sequencing technologies combine to provide a new method for the analysis of FFPE samples.