Antiproliferative Effect of Lung Cancer Cell Lines and Antioxidant of Macluraxanthone from Garcinia Speciosa Wall

Abstract Garcinia speciosa Wall.is Thai medicinal plant used as remedies for healing skin infections and wounds, a laxative (bark), inflammation, astringent (leaves) agents, diarrhoea (fruits) and antibacterial.This plants were rich source of oxygenated xanthones, some of which have antitumor promotive activity. Dried leaves and twigs of G. speciosa extracted with ethanol and chromatography with Diaion20 column by H 2 O, methanol and ethyl acetate gave 3 fractions. The crude extracted of H 2 O, methanol and ethyl acetate fractions were tested by cytotoxic against to the lung cancer cell lines (A 549) gave ED 50 values of 62, 45 and 75 μg/ml, respectively. Hence, the crude methanol fraction was interested for chemical constituents and identified cytotoxic activities. Crude methanol fraction was fractionated by si gel column, used of ethyl acetate-hexane (7:3) gave macluraxanthone was major compound. Structure elucidated by 1H, 13C and 2D NMR spectroscopy. The antioxidant of macluraxanthone by DPPH showed IC 50 was 7.65 μg/ml and the cytotoxic test of lung cancer cell lines (A 549) gave ED 50 values 15.38 μg/ml.