INHIBITION OF SERINE BETA -LACTAMASES BY ACYL PHOSPH(ON)ATES : A NEW SOURCE OF INERT ACYL AND PHOSPHYL ENZYMES
1998
Acyl phosph(on)ates are shown to inhibit serine β-lactamases and provide a new source of relatively stable complexes. Thus, benzoyl phenyl phosphate, benzoyl phenylphosphonate, and dibenzoyl phosphate react with the class C β-lactamase of Enterobacter cloacae P99 at micromolar concentrations to form an acyl enzyme of half-life about 40 s. The phosphonate reacts further more slowly to produce a much more inert complex. Dibenzoyl phosphate reacts with the class A TEM β-lactamase to form an acyl enzyme of half-life about 8 s and, more slowly, reaching completion after an average of about 80 turnovers, a more inert complex, of half-life about 2 h. The acyl phosphonates thus represent a new starting point for the design of β-lactamase inhibitors and perhaps of antibacterial agents.
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