A novel regulatory function of proteolytically cleaved VEGF-2 for vascular endothelial and smooth muscle cells.
1997
By high throughput sequencing, we have identified a cDNA encoding a polypeptide related to vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) in the VEGF/PDGF gene family. It is designated vascular endothelial growth factor 2 (VEGF-2). Similar to VEGF, expression of VEGF-2 mRNA is abundant in vascular smooth muscle cells and several highly vascularized tissues. VEGF-2 protein is expressed as a secreted 52 kDa precursor as well as the 30 kDa amino-terminal and 27 kDa carboxy-terminal cleavage products. The latter two cleavage products are linked via a disulfide bridge (or bridges) and can be copurified. Using copurified 30 and 27 kDa proteins, the effect of VEGF-2 on growth of several cell types, including vascular endothelial and smooth muscle cells, was determined. Our results demonstrate that VEGF-2 protein stimulates the growth of human vascular endothelial cells but inhibits growth of human aortic smooth muscle cells induced by platelet-derived growth factor. These studies est...
Keywords:
- Vascular smooth muscle
- Vascular endothelial growth factor C
- Vascular endothelial growth inhibitor
- Growth factor
- Vascular endothelial growth factor A
- Vascular endothelial growth factor
- Myocyte
- Biology
- Molecular biology
- Vascular endothelial growth factor B
- Platelet-derived growth factor receptor
- Complementary DNA
- Gene family
- Correction
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