Construction and Characterization of crp~-/gfp~+ Mutant of Salmonella choleraesuis C500 Strain

Salmonella choleraesuis C500 strain crp-/gfp+ mutant obtained by deleting crp gene and inserting gfp gene was constructed. Firstly, the recombination suicide vector with 320 bp-deleted crp gene and 720 bp-insert gfp gene was constructed and conjugated with C500. The unmarked crp deleted and gfp inserted strain was selected by a two-step method. The crp deletion and gfp insertion on the genome were determined by PCR, sequencing and fluorescence observation with microscope, indicating that the crp-/gfp+ mutant was successfully constructed.