The effect of PMMA bone cement particles on the expression of SOCS1 and SOCS3 genes in rat macrophages

Objective To observe the effect of PMMA bone cement particles on the expressions of SOCS1 and SOCS3 genes by rat macrophages in vitro.Methods Macrophages were obtained from the peritoneal of rats and cultured.Cell purity was identified with immunocytochemistry using monoclonal antibody against CD68.Real-time PCR method was used to detect the expression levels of SOCS1 and SOCS3 genes.The effects of PMMA bone cement particles on the expression of SOCS1 and SOCS3 genes were observed at different concentrations and time.Results PMMA bone cement particles increased the expression of SOCS1 and SOCS3 in cultured macrophages,with a certain time-and dose-dependent fashion.The expression of SOCS1 and SOCS3 increased significantly at a concentration of 0.25 mg/ml of PMMA(P0.05),and elevated the most significantly in cultures at 24h(P0.05).Conclusion PMMA bone cement particles can stimulate the expression of SOCS1 and SOCS3 in macrophages.