The role of hyaluronic acid and amino acid against the aging of the human skin: a clinical and histological study.

BACKGROUND In aesthetic medicine different techniques have been used against the aging of the human skin especially in the facial area. Hyaluronic acid is used for improving the quantity of water and extracellular matrix molecule. The aim of this study is a clinical and histological evaluation of the effect of low molecular weight hyaluronic acid fragments mixed with amino acid (HAAM) on the rejuvenation the face skin treated with intradermal microinjections. METHODS Twenty women with mean age 45 between range 35-64 were studied, there of 8 in menopause and 12 of childbearing age. The patients were treated with the HAAM products by mesotherapy technique; before and after three months of the therapeutic procedure each patient underwent small biopsies with a circular punch biopsy. RESULTS The clinical results of the present study showed, that the administration of the dermal filler containing fragments of hyaluronic acid between 20 and 38 monomers and amino acid via dermis injection technique produces an aesthetic improvement in the faces of the treated patients, while the histological evaluation shows an increased fibroblast activity with the production of Type III reticular collagen and increased number of vessels and epidermis thickness. CONCLUSIONS The clinical and histological assessment showed that subcutaneous HAAM infiltration have a significant impact on the dermis and clinical aspects of the face.