An in situ assembly strategy for the construction of a sensitive and reusable electrochemical aptasensor

The 5′-end thiolated aptamer is attached on the electrode surface through hybridization with immobilized assistant strands. Then the signal enhancing platform of gold nanoparticles and an electroactive Cu2+–L-cysteine tag are in situ tethered on the 5′-end of the aptamer, through which, a sensitive and reusable aptasensor is facilely constructed.