The influence of the temperature in the UV-Vis spectrophotometers calibration

The study of the influence of temperature as a variable during the spectrophotometer calibration is very important to many laboratories, due to the fixed temperature imposed by the national laboratory (CENAM). This gives us an invaluable insight to know in ahead that we do not have to be tied up to a unique temperature inherited by CENAM. In this manner we can increase our scope in which we can accomplish a trustful metrological calibration in a wide temperature range. For this reason, in this work a study of the temperature influence in the calibration of swept UV-Vis spectrophotometers is shown. The temperature interval was set between 18°C to 31 °C, and glass filters of neutral density at 1%, 50%, and 90% were used as a reference approved materials to calibrate the photometric scale and a holmium oxide glass filter was used to calibrate the wavelength scale. An ANOVA analysis was used (variance analysis) and the obtained results have a confidence of 95,45%. The results of this study show that the temperature is not an influence variable during the spectrophotometer calibration in the interval of 18°C to 31°C, which allows us to simplify the uncertainty estimation model.