Preparation of a hybrid organic-inorganic monolith for extraction and purification of quercetin and myricetin from Chamaecyparis obtusa

A new hybrid organic-inorganic monolithic cartridge was synthesized and used as the selective sorbent for the extraction and purification of quercetin and myricetin from Chamaecyparis obtusa via a solid-phase extraction method. The morphology of the monolithic material was examined by field emission-scanning electron microscopy and a Brunauer-Emmett-Teller(BET) test. The adsorption capacity of the obtained material for quercetin and myricetin was investigated by fitting the adsorption data to four different adsorption equations, of which the Langmuir-Freundlich isotherm was selected as the most suitable model. Under optimized conditions, good calibration curves were observed at nine concentrations ranged from 0.5 μg/mL to 100.0 μg/mL of quercetin and myricetin. The extraction recovery ranged from 74.5% to 84.6% and the inter- and intra-day relative standard deviations were <6%. This type of hybrid monolith has potential for the separation and purification of bioactive compounds from natural plant extracts.