Molluscicidal activity of endophyte JJ18 from Pseudolarix kaempferi inoculated to beach soil.

Endophyte JJ18 from Pseudolarix kaempferi was inoculated on solid medium (A), sterile soil (B), and non-sterile soil (C), respectively. The molluscicidal efficacy of fermented products of soil extract was detected according to the immersion test method recommended by WHO, the survival time of JJ18 was observed. Control groups included non-sterile soil (D), ionic water without chloride ion (E) and niclosamide (F). The acute toxicity of A-D group against Artemia salina was determined with artificial seawater as control. After immersing for 24 h, 48 h and 72 h, the snail mortality of group B [(36.67±7.56)%, (63.33±4.65)%, and (90±0)%] was significantly higher than those of group D [(30±6.87)%, (33.33±5.68)%, and (56.67±8.55)%] (P0.05, P0.01). Endophyte JJ18 survived in soil for at least 30 days. The mortality of Artemia salina in group B [(25.24±3.74)%] was significantly lower than that of group A[(57.15± 8.90)%] (P0.01).