Explore the difference between the single-chamber and dual-chamber microbial electrosynthesis for biogas production performance

Abstract Microbial electrosynthesis (MES) is an advanced technology for efficient treatment of organic wastewater and recovery of new energy, with the advantages and disadvantages of single-chamber and dual-chamber MES reactors being less understood. Therefore, we explored the effects of single-chamber and dual-chamber structures on the methane production performance and microbial community structure of MES. Results indicated that methane concentration and current density of single-chamber MES were higher than those of dual-chamber MES, and the system stability was better, while chemical oxygen demand (COD) removal rate and cumulative methane production were not significantly different. Analysis of microbial community structure showed the abundance of acidogens and H2-producing bacteria was higher in single-chamber MES, while fermentation bacteria and methanogens was lower. The abundance of methanogens of dual-chamber MES (21.74–24.70%) was superior to the single-chamber MES (8.23–10.10%). Moreover, in dual-chamber MES, methane was produced primarily through acetoclastic methanogenic pathway, while in single-chamber MES cathode, methane production was mainly by hydrogenotrophic methanogenic pathway. Information provided will be useful to select suitable reactors and optimize reaction design.