Endogenous nutriment variation in cuttings of Thuja occidentalis during the formation of adventitious root

Thuja occidentalis was taken as the experimental material and cuttings were treated by ABT2,GGR6 and water in order to study the endogenous nutriments variation in softwood cuttings during the formation of adventitious root.The results showed that the changes of the total soluble sugar contents were similar in the cuttings treated by plant growth regulators and water,and the total soluble sugar contents reached peaks in induction period and elongation period,respectively.Yet the total soluble sugar content in the cuttings treated by ABT2 was relatively high and that by water was relatively low,which meant that the higher total soluble sugar content could promote rooting.The nitrogen contents in the cuttings treated by plant growth regulators and water were decreased,and the nitrogen content treated by water was higher than that treated by plant growth regulators,which meant that the lower nitrogen content might be more conducive to root.The change trends of the C/N value were consistent with the change trends of total soluble sugar.The grey incidence analysis showed that the total soluble sugar and C/N value were the major factors affecting rooting,which was more obvious in induction period and elongation period.