Electron tomography & template matching of biological membranes
2008
In recent years, electron tomography has proven to be a valuable addition to existing EM techniques, providing new and exiting insights into cellular architecture in three dimensions. Constant technical developments have played a crucial role in establishing the role of the technique in modern electron microscopic studies. The next technical bottleneck to overcome is that of a reliable, objective, and (semi)-automatic analysation of the acquired data. One such approaches is three dimensional template matching based on normalized cross-correlation analysis (figure 1) [1].
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