Effects of 5-hydroxydecanoate on Kv1.5 expression in pulmonary arteries of chronic hypoxic rats

AIM:To investigate the effect of mitochondrial ATP-sensitive potassium channel(mitoKATP) inhibitor 5-hydroxydecanoate(5-HD) on chronic hypoxic pulmonary artery hypertension(CHPAH) in rats.METHODS:Twenty-four male Sprague-Dawley rats were randomly divided into 3 groups:control group,hypoxia group and hypoxia+5-HD group.To establish an animal model of CHPAH,the rats in hypoxia group and hypoxia+5-HD group were placed into a hypoxic and normobaric chamber [O2(10.0±0.3)%] for 4 weeks(8 h a day,6 days a week).The mean pulmonary arterial pressure(mPAP) and the ratio of right ventricle weight to left ventricle with septum weight [RV/(LV+S)] were measured.Western blotting and reverse transcription-polymerase chain reaction(RT-PCR) were performed to analyze the protein and mRNA levels of Kv1.5 in the pulmonary artery,respectively.RESULTS:The mPAP and RV/(LV+S) were much higher in hypoxia group than those in control group,while those in hypoxia+5-HD group were decreased as compared to hypoxia group.The protein and mRNA levels of Kv1.5 in hypoxic group were significantly lower than those in control group,and this decrease was partly reversed by 5-HD.CONCLUSION:5-HD may exert a protective effect on CHPAH by decreasing mPAP and RV/(LV+S).Inhibiting mitoKATP and stimulating Kv1.5 expression by 5-HD may contribute to the mechanism of this effect.