Penetration of zona-free hamster ova by Siberian tiger sperm

Sperm from the electroejaculate of captive Siberian tigers (Panthera tigris altaica) penetrated zona pellucida-free hamster ova in vitro, evidenced by a decondensation reaction. This assay, when used in conjunction with semen analysis, may be useful in assessing the fertility potential of males of this and other related felids. Such information is an important step in developing successful long-term management strategies for captive and wild populations of this severely endangered species.