New semen freezing method for chicken and duck using dimethylacetamide as the cryoprotectant

ABSTRACT The aim is to optimize the dimethylacetamide (DMA) straw freezing technology through the handy patent equipment, screening the formula of basic extender for chicken semen freezing and optimizing the DMA addition method, and then by comparing the fertility of DMA straw frozen semen with the pellet frozen semen. After the DMA straw freezing technology is optimized, it is extended to duck semen. In different extenders, the frozen sperm motility of Lake and Ravie (LR) group is 64%, the fertility 49.57% and the hatchability 91.52%, all of which are superior to those of FEB, Beltsville Poultry Semen Extender (BPSE) and Lake (P 0.05). The fertility and hatchability of DMA straw group are 77.61% and 92.30%, respectively, and it is significantly higher than those in the pellet group (P