An efficient protocol for in vitro regeneration of Podophyllum hexandrum, a critically endangered medicinal plant.

Rhizome explants were used to develop a protocol for in vitro plantlet regeneration of Podophyllum hexandrum , a critically endangered medicinal plant, through direct organogenesis. Highest rate of multiple shoot formation was noted in MS medium supplemented with 11.42 µM IAA within three months. A synergistic effect of 2.68 µM NAA and 11.1 µM BAP was second best. The ½ MS liquid medium with 100 µM IBA was most suitable for rooting of shoots. Leaf explants resulted in callus formation only. Leaf regenerated calli showed the presence of podophyllotoxin in HPLC analysis.