БИВАЛЕНТНАЯ КОМПОЗИЦИЯ РЕКОМБИНАНТНЫХ ШТАММОВ ВИРУСА ГРИППА, ЭКСПРЕССИРУЮЩИХ БЕЛКИ РАЗЛИЧНЫХ ФАЗ ЖИЗНЕННОГО ЦИКЛА MYCOBACTERIUM TUBERCULOSIS, ДЛЯ ПРОФИЛАКТИКИ ТУБЕРКУЛЕЗА ЛЕГКИХ

The efficacy of immunization against tuberculosis with vaccine candidates based on attenuated recombinant influenza vectors expressing mycobacterial proteins 67TB10.4, Ag85B and HspX in different combination was tested in C57black/6 mice. The greatest effectiveness of preventive immunization against tuberculosis was shown for the bivalent vaccine composition Flu/TB10.4_HspX ++ Flu/Ag85B. Double intranasal immunization with this composition provided significantly greater protective effect than one BCG immunization in mice 6 weeks after intravenous M. tuberculosis Erdman challenge. Vaccination with Flu/TB10.4_HspX + Flu/Ag85B decreased bacterial load in the lungs (+ 0.69 lg CFU) and spleen (+0.67 lg CFU) compared with the BCG vaccinated group resulting in a greater delay prevalence of tuberculousinflammation and alteration in the lungs. In comparison with the effect of BCG the immunization with Flu/TB10.4_HspX + Flu/Ag85B also provided activation of the lymphoid component of the cellular inflammatory response according to registration of lymphoid granulomas and large lymphohistiocytic infiltrates and complete inhibition of the absorption and digestion activity of peritoneal macrophages, which is typical for tuberculosis infection in mice.