An Introduction to Design of Microbial Strain using Synthetic Biology Toolboxes for Production of Biomolecules

Metabolic engineering has the potential to produce a wide range of biomolecules from renewable resources through the extension or modification of natural biosynthetic pathways in the microbes. Over the past two decades, it has been used for the production and optimization of number of biomolecules that can be easily tuned or regulated by synthetic biology and molecular biology toolboxes. Random mutagenesis or targeted genetic or genome engineering tools allow industrial strains to improve the productivity and yield of biomolecules or metabolites. In this chapter, we highlight the recent advances, challenges and future perspective in metabolic engineering towards industrial-scale production of biomolecules.