In vitro mutagenesis protocols

In this volume experts from industrial and academic laboratories describe easily reproducible and up-to-date procedures for site directed and random mutagenesis. Site-directed protocols include those based on strand selection, PCR (including "splicing by overlap extension" and the "megaprimer" procedure), the ligase chain reaction, positive antibiotic selection, unique restriction site elimination, gapped heteroduplex formation, and solid-phase capture with the biotin/strepavidin system. Many can be used with virtually any double-stranded DNA plasmid. The bookcovers genesis, linker scanning mutagenesis, nested deletion mutagenesis, and a specialized E coli mutator strain.