RNA-Seq and its applications in leukemia and lymphoma
2014
The transcriptome is the complete set of transcripts for certain type of cells or tissues in a specific developmental stage or physiological condition.Transcriptome analysis can provide a comprehensive understanding of molecular mechanisms involved in specific biological processes and diseases from the information on gene structure and function.RNA-seq,refers to the use of next-generation highthroughput sequencing technologies to sequence cDNA library transcribed from all RNAs in tissues or cells,can be used to quantify,profile,and discover RNA transcripts by Sequencing,assembling,mapping reads.RNA-Seq,as a new efficient and fast transcriptome sequencing technology,has been widely used in biological,medical research.The detailed principles,technical characteristics and applications of RNA-seq in leukemia and lymphoma are reviewed here.
Key words:
Transcriptome sequencing (RNA-Seq) ; Leukemia ; Lymphoma
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