Evaluation of acute oral toxicity of methanolic fractions of Sesbania grandiflora Linn., roots in albino mice

Introduction: Although Sesbania grandiflora Linn. roots have valuable pharmacological effects, the comprehensive awareness about its toxicity potential has been lacking. Objectives: To assess the acute oral toxicity of methanolic fraction of Sesbania grandiflora root (MFSGR). Methods: The female albino mice were divided into two groups (n=5), one group served as normal control while the other treated with MFSGR (2000 mg/kg, p.o). Both the groups of mice were observed for 14 days for various parameters according to OCED guidelines 425 TG. Results: On oral administration of MFSGR at a dose of 2000 mg/kg did not resulted in mortality and treatment-related adverse reactions such as variation in water and food consumption, alteration in body weight, relative organ weights, hematological and biochemical parameters. However, treatment showed mild changes in behavioural patterns like sleeping and itching during initial time point. Further, histopathological evaluation of vital organs exhibited normal architecture and this observation provided good support to general and biochemical measurements. Conclusion: The present study revealed that the methanolic fraction of S. grandiflora did not show any signs of toxicity in female albino mice. Hence the methanolic fraction can be used for the pharmacological studies by calculating dose based on toxicity studies. ;TJPS-2020-0026.R1