A Mutant of PlxyCSP1 Protein in Plutella xylostella

[Objective] This study aimed to explore the effects of cysteine(Cys58)on the characteristics of PlxyCSP1 in P.xyostella combined with pesticide compounds.[Method] Cys58 of PlxyCSP1 was mutated into Trp58 by using overlap extension PCR method,and PlxyCSP1-M2 mutant was obtained.Expression vector was constructed and the protein was detected by western blot.[Result] Expression vector pET32a-PlxyCSP1-M2 was constructed to express the 35 kDa weight protein.[Conclusion] PlxyCSP1 mutant protein was expressed successfully in prokaryotic expression system.