Mutation detection by solid-phase chemical cleavage of mismatches using radioactive probes.

This protocol is the first of two describing solid-phase chemical cleavage of mismatch (SpCCM), a method for the detection of mutations in genomic DNA. DNA fragments up to 2 kb in length can be analyzed to determine the position (within 10-15 bp) and identity of the mismatched nucleotide. While this protocol uses radioactively labeled probes and conventional gel electrophoresis followed by autoradiography, the second protocol employs fluorescently labeled primers and depends on an automated electrophoresis system with fluorescence detection Mutation Detection by Solid-Phase Chemical Cleavage of Mismatches Using Radioactive Probes.