A kind of orientation coupling method and the application of microballoon and antibody

The invention discloses the orientation coupling method of a kind of microballoon and antibody, carboxyl microballoon realizes orientation coupling with antibody F (ab ') segments in such a way that sulfydryl is coupled, and forms antibody microsphere compound.This method can effectively control direction when antibody is connect with microballoon, realize orientation coupling to improve sensitivity of the latex enhancing immune than turbid reagent, and can improve anti-interference ability of the reagent for rheumatoid factor (RF).