Transgenic regulation and control miRNA (micro ribonucleic acid) gene algae capable of realizing continuous photosynthetic hydrogen production and creation method of gene algae

2013 
The invention discloses transgenic regulation and control miRNA (micro ribonucleic acid) gene algae capable of realizing continuous photosynthetic hydrogen production. Regulation and control miRNA is screened from a green algae miRNAs library or is designed for a green algae functional gene affecting photosynthetic efficiency, transgenic algae capable of inducing the expression of the regulation and control miRNA through such manners as light intensity or temperature and the like is created, and the activity of a green algae photosynthetic system II is indirectly regulated and controlled by means of inhibition of the miRNAs on a target gene to control the hypoxia environment in algae cells so as to enable the green algae cells to continuously produce hydrogen; a culture medium does not need to be alternately changed in the hydrogen production process of the transgenic regulation and control miRNA gene algae created by the method, and thus the defect that the culture medium needs to be alternately changed to realize the continuous hydrogen production of the existing green algae to cause great reduction on the growth and hydrogen production ability of the culture medium is overcome, and the transgenic regulation and control miRNA gene algae has an industrial application prospect.
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