Cultured fucosidosis fibroblasts: a simple technique demonstrating storage of tritiated-fucose labeled material.
2008
Incorporation of tritiated fucose into the acid-soluble and acid-insoluble fractions of cultured fucosidosis and control fibroblast monolayers was determined over a 10-day period. Fucosidosis cells incorporated significantly more label into the acid-soluble, but not the acid-insoluble fraction, than control cells. The ratio of acid-soluble to acid-insoluble radioactivity increased linearly from 1 to 10 days for the fucosidosis cells, indicating the storage of low molecular weight partial degradation products.
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