Extraction and analysis of polysaccharide from Momordica charantia

Abstract Momordica charantia is a kind of medicine and food homologous fruit. The M. charantia polysaccharide has the physiological functions of clearing away heat and detoxification, lowering blood sugar, improving immunity and so on. It is an ideal health care product.MP3 from M. charantia polysaccharides were isolated and purified by DEAE cellulose column chromatography and gel column chromatography. The composition of polysaccharides was determined by thin layer chromatography (TLC) and gas chromatography, and the relative molecular weight was determined by gel filtration. The results showed there were typical absorption peaks of polysaccharide in its infrared spectrum, and there were no characteristic absorption peaks of nucleic acids and proteins in its ultraviolet scanning. MP3 contained rhamnose, xylose, galactose and arabinose, and its average molecular weight was 9.57 × 104. This method was easy to operate and laid a foundation for the development of M. charantia polysaccharide products.