Study on Experimental Factors of Passive Cutaneous Anaphylaxis in Rats

Objective:To investigate the factors of passive cutaneous anaphylaxis(PCA) in rats to select experimental conditions and improve the accuracy of forecasts for experiments.Method:Egg albumin and bovine serum albumin were selected as test antigens for the PCA.PCA experiments were carried out in different experimental conditions(with or without kasa agents,allergens means,rat strains,the optimal time for preparing antiserum of blood withdrawn,the difference in immunogenicity of egg albumin and bovine serum albumin,etc.).Result:① Adjuvant could significantly enhance the sensitivity of immune response.in SD and Wistar tast.② Intraperitoneally and subcutaneously injection sensitization both could get good response of PCA,but intraperitoneally injection with adjuvant could induce chronic inflammation of abdominal cavity.③ Sensitivity of immune response to antigen was similar in SD and Wistar rats.However,to use different strains of rats with PCA were better than to use single stain of experimental rats.④ Content of IgE in serum after 16 days sensitization was higher than that in 14 days sensitization.⑤ Egg albumin was superior to bovine serum albumin in immunogenicity of PCA test.