Exosomes derived from miR-129-5p modified bone marrow mesenchymal stem cells represses ventricular remolding of mice with myocardial infarction

Background Myocardial infraction (MI) is a severe disease with great mortality. Mesenchymal stem cells (MSCs)-derived exosomes display protection against MI. MicroRNA-129-5p was reported to exert anti-inflammation activity by targeting high mobility group box 1 (HMGB1). In the present study, the effects of MSCs-derived exosomes overexpressing miR-129-5p on MI were evaluated. Methods Bone marrow mesenchymal stem cells (BMSCs) were transfected with miR-129-5p for exosomes isolation. MI mice model was established and administrated exosomes overexpressing miR-129-5p. The cardiac function, expression of HMGB1,inflammatory cytokines, apoptosis and fibrosis in heart tissues were measured. Resutls MiR-129-5p inhibited HMGB1 expression in BMSCs. MI mice treated with exosomes overexpressing miR-129-5p had enhanced cardiac function and decreased expression of HMGB1 and production of inflammatory cytokines. Exosomes overexpressing miR-129-5p further prevented apoptosis and fibrosis. Conclusion Exosome-mediated transfer of miR-129-5p suppressed inflammation in MI mice by targeting HMGB1. This article is protected by copyright. All rights reserved.