Non-invasive imaging of zebrafish with spinal deformities using optical coherence tomography: a preliminary study
2013
A zebrafish model has recently been introduced to study various genetic mutations that could lead to spinal deformities
such as scoliosis. However, current imaging techniques make it difficult to perform longitudinal studies of this condition
in zebrafish, especially in the early stages of development. The goal of this project is to determine whether optical
coherence tomography (OCT) is a viable non-invasive method to image zebrafish exhibiting spinal deformities. Images
of both live and fixed malformed zebrafish (5 to 21 days postfertilization) as well as wild-type fish (5 to 29 days
postfertilization) were acquired non-invasively using a commercial SD-OCT system, with a laser source centered at
930nm (λ=100nm), permitting axial and lateral resolutions of 7 and 8μm respectively. Using two-dimensional images
and three-dimensional reconstructions, it was possible to identify the malformed notochord as well as deformities in
other major organs at different stages of formation. Visualization of the notochord was facilitated with the development
of a segmentation algorithm. OCT images were compared to HE histological sections and images obtained by calcein
staining. Because of the possibility of performing longitudinal studies on a same fish and reducing image processing
time as compared with staining techniques and histology, the use of OCT could facilitate phenotypic characterization in
studying genetic factors leading to spinal deformities in zebrafish and could eventually contribute to the identification of
the genetic causes of spinal deformities such as scoliosis.
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