Brugia pahangi: Stage-specific antigens on microfilariae detected by serum from infected jirds or by monoclonal antibodies

Abstract Experiments were carried out to determine whether there are stage-specific antigens on microfilariae of Brugia pahangi , using sera from Mongolian jirds infected with B. pahangi and monoclonal antibodies against microfilariae of B. pahangi . These studies showed that microfilariae have both stage-specific and nonspecific antigens. The nonspecific antigens were also present on adult worms and on infective larvae. Among monoclonal antibodies, 6 out of 14 clones produced antibodies against the microfilarial stage-specific antigens, and 8 clones produced antibodies against nonspecific antigens. These monoclonal antibodies could not distinguish between adults, microfilariae, or infective larvae of B. malayi and B. pahangi .