Monoclonal antibodies to cytoplasmic tetrodotoxin-sensitive brain protein. Effects of veratrine on binding between antibodies and neuroblastoma cells

The effects were studied of neurotoxins, which modulate the activity of voltagedependent sodium channels, on binding between neuroblastoma cells and monoclonal antibodies to cytoplasmic tetrodotoxin-sensitive protein displaying the properties of tetrodotoxin-sensitive sodium channles when incorporated into the liposomes. Binding between antibodies and the monolayer of viable (unfixed) cells recorded by immunoenzyme testing was found to break down in the presence of veratrine (veratridine). It is postulated that the antibodies obtained bind with an antigenic determinant located at or near the veratrine binding site.