[Investigation of the distribution of (±)-N-methyl-3-phenyl-3-(para-trifluoromethyl) phenoxypropylamine hydrochloride in the body of warm-blooded animals].

THE AIM OF THE STUDY Was to investigate the distribution of (±)-N-methyl-3-phenyl-3-(para-trifluoromethyl) phenoxypropylamine hydrochloride (fluoxetine) in the body of warm-blooded animals. For the experiments, Wistar rats were used. Fluoxetine was isolated from animal organs and bio-liquids by liquid-liquid extraction. Thin layer chromatography, UV spectrophotometry, and high performance liquid chromatography were used for identification and quantification. Techniques for isolating fluoxetine from urine, blood, intestinal contents, kidneys, and liver samples using liquid-liquid extraction are presented. Methods for identification and quantitative determination of fluoxetine in extracts by thin layer chromatography, UV spectrophotometry, and high performance liquid chromatography are described. The greatest amount of fluoxetine was found in the kidneys - 12.94±2.18 mg/100 g, the small intestine with contents - 9.50±1.90 mg/100 g and liver - 9.28±1.37 mg/100 g.