An Agile Pipeline for RNA-Seq Data Analysis

Next-generation sequencing provides a more efficient way to characterize the transcriptome; meanwhile, it introduces significant issues for the effective analysis of large-scale sequencing data. To counteract this growing problem, we developed a robust and modular pipeline for examining whole transcriptome sequencing data quickly. Utilizing a Docker for containerization and unanimous cross-platform support, we modularized the pipeline and created a practical, automated structure for pragmatic use. Furthermore, we have developed segregated implementations of the pipeline sections to allow end-users to craft custom pipelines easily. We applied our tool to various datasets and showed that our pipeline offered high-quality sequencing data analysis.