DNA Typing of Coagulation Factor XIII “a” Subunit by PCR-RFLP and SSCP

Data on nucleotide sequences of conventional polymorphic markers leads to development of PCR-based typing methods such as PCR-RFLP, PCR-ASO, or SSCP. RFLP usually requires loss or gain of a recognition sequence of a restriction enzyme in which a polymorphic site is included, but artificially introduced mismatch of a single nucleotide in a PCR primer can produce a recognition site of a restriction enzyme (Kumar and Dunn 1989).