Comparative Proteomic Analysis Reveals Complex Responses to Bordetella bronchiseptica Infections in the Spleen of Rabbits.

Bordetella bronchiseptica (B. bronchiseptica) is a gram-negative bacterium that causes a respiratory disease in rabbits. To determine the proteins of B. bronchiseptica in rabbits related to the disease, differentially accumulated proteins in B. bronchiseptica-infected cells were identified by comparative proteomic analysis. After challenge for 7 days, spleens in infected rabbits were found to be enlarged than spleens of uninfected rabbits. Comparative proteomic analysis detected 5814 proteins and quantified 4854 of these. Fifty eight upregulated and 38 downregulated proteins were identified in spleen tissue after B. bronchiseptica infection of rabbits (both p<0.05). The significantly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were ribosome, biosynthesis of amino acids, biosynthesis of amino acids, protein export, carbon metabolism, and glycine, serine, and threonine metabolism (all p<0.01). Significantly enriched KEGG pathways included 'ocu03010 ribosome' (a); 'ocu00260 glycine, serine threonine metabolism'. Analyses of control and infected spleen cells, detected responses to B. bronchiseptica infection. Many differentially affected proteins were evident, and reflected different biological changes and diverse subcellular localizations between control and infected spleen cells. Infection markedly altered the expressions of proteins linked to the serine protease system, with the 'phagosome,' 'biosynthesis of amino acids,' 'glycine, serine threonine metabolism,' 'intestinal immune network for IgA production', and 'amino sugar and nucleotide sugar metabolism' associated with B. bronchiseptica infection. The result will inform studies of responses to B. bronchiseptica infections in rabbits. This article is protected by copyright. All rights reserved.