Urease activity and microbial biomass of carbon in hydrocarbon contaminated soils. A case study of cheshmeh-khosh oil field, Iran.

Abstract Crude oil contamination in soils may result in destructive effects on soil microorganisms and plants and act as a source of groundwater contamination. The objective of this study was to evaluate the biological activities for a better understanding of ecological risks. A couple of biological assays, including soil microbial biomass of carbon (SMBC) and urease activity (UA), were used to evaluate the microbial activities in soils. The chemical analysis demonstrated different values of total petroleum hydrocarbons (TPHs) concentrations (from 0.12 to 2.99 mg/kg of dry soil) and relatively high quantities of Nickel (from 32 to 136.8 mg/kg of dry soil) and cadmium (from 0 to 4 mg/kg of dry soil) in samples. UA and SMBC values were comparatively lower in close distances to oil wells, pipelines, and especially drilling sediments pool. The spatial variability maps using the interpolation module by GIS specified the line from northwest to the southeast of the area as a more affected area by TPHs and N i + C d .