Immobilization of glucose isomerase

Immobilized glucose isomerase is prepared by mixing 0.5 to 1.5 parts by weight whole microbial cells containing glucose isomerase with one part by weight agar, combining the resultant mixture with an organic solvent, recovering discrete particles of agar gel with the whole microbial cells entrapped therein and drying the agar gel particles. The immobilized glucose isomerase has long half life stability when used in a column in a continuous process to convert glucose to fructose.