1,5-二芳基-3-（2-羟基-4,6-二甲氧基苯基）-2-吡唑啉的合成及铜离子荧光探针行为

The design and synthesis of chemosensors for Cu(superscript 2+) constitute a very active area of research as a result of the demand for more sensitive and selective chemosensors for in vitro and in vivo purposes. The synthetic Cu(superscript 2+) selective fluoroionophores so far reported suffer from low sensitivity and, in general, higher concentrations of interfering metal ions disturb the selectivity towards Cu(superscript 2+). Four 1,5-diaryl-3- (2-hydroxyl-4,6-dimethoxylphenyl) -2-pyrazoline (4a-4d) were synthesized and fluorescent sensors for Cu(superscript 2+) ion were investigated. The structures of all the products were determined by IR, 1H NMR, MS and elemental analysis. Their UV and fluorescence spectra were measured. The influences of eight metal ions such as Cu(superscript 2+), Zn(superscript 2+), Co(superscript 2+), Ni(superscript 2+), Na(superscript +), K(superscript +), Ca(superscript 2+) and Mg(superscript 2+) on their fluorescence properties were studied. Spectroscopic studies revealed that the compounds 4a-4d had rather strong affinity toward Cu(superscript 2+). The specificity of the probes 4a-4d toward Cu(superscript 2+) were determined. Nearly no fluorescence intensity changes were observed in emission spectra with Zn(superscript 2+), Co(superscript 2+), Ni(superscript 2+), Na(superscript +), K(superscript +), Ca(superscript 2+) and Mg(superscript 2+). However, under identical conditions, the results show fluorescence quenching with Cu(superscript 2+). So we have developed four new fluorescent sensor 4a-4d. They show high sensitivity and selectivity toward Cu(superscript 2+) in DMSO.