Transforming growth factor type beta 3 maps to human chromosome 14, region q23-q24.

Abstract Type beta transforming growth factors (TGF-betas) are polypeptides that act hormonally to control the proliferation and differentiation of multiple cell types. Recently, we reported the isolation of a cDNA encoding a new member of this gene family, which we have termed TGF-beta 3. Here we show by Southern analysis using a human probe specific for TGF-beta 3, the presence of a related single copy gene in a wide range of animal species. Chromosomal localization of the TGF-beta 3 gene was performed by Southern blot analysis of DNA prepared from 24 human-Chinese hamster somatic cell hybrids using a specific TGF-beta 3 cDNA probe. The human specific restriction fragments segregated only with human chromosome 14. For all other human chromosomes high discordancy scores were obtained. Using in situ hybridization of human metaphase chromosomes, the regional location could be identified. Hybridization of the TGF-beta 3 cDNA probe resulted in specific labeling of chromosome 14, bands q23-24.