Transformation of large DNA fragment of Panax ginseng into Ganoderma lucidum

A 100kb DNA fragment of Panax ginseng cloned in a binary bacterial artificial chromosome (BIBAC) vector was transformed into protoplasts of Ganoderma lucidum by electroporation. The results showed that a number of regenerative transformants were obtained on the selective medium when 5μL of the Panax ginseng large DNA fragment solution was transformed into 75μL of the Ganoderma lucidum protoplasts using 4mm spaced electrodes at 240 volts. The transformants were analyzed by PCR using two pairs of primers designed from two flanking sequence of the vector cloning site. The results indicated that the ginseng large DNA fragment had been transformed into the Ganoderma lucidum genome.