NONSTEROIDAL ANTI-INFLAMMATORY DRUGS INDUCE ADIPOGENESIS IN MURINE PLURIPOTENT STEM CELLS

Introduction: Studies have shown steroidal and non-steroidal anti-inflammatory drugs (NSAIDs) suppress bone remodeling. Previous results have indicated that NSAIDs suppress proliferation and induce cell death in cultured osteoblasts and pluripotent stem cells (D1-cells), suggesting these effects might be one of the mechanisms contributing to their inhibitory effects on bone remodeling in vivo. On the other hand, our previous results indicated that dexamethasone treatment shifts the characteristics of osteogenesis into adipogenesis in D1-cells. However, the influences of NSAID on adipogenesis in pluripotent stem cells have rarely been investigated. In this study, we tested the adipogenesis of D1-cells upon long-term treatment of NSAIDs. NSAID influence on the osteocalcin expressions of D1-cells was also examined.Materials and Methods: The effects of treatments with indomethacin, ketorolac, diclofenac and piroxicam (10−5 and 10−4 M) for 2, 4 6 or 8 days were evaluated. Lipid droplets in cultures were detect...