Articles having disposed thereon and production methods thereof localized molecules

A method of providing a substrate having an enzyme selectively immobilized on it, comprendiendoel method comprising: providing a substrate having a first surface component comprising SiO2 and segundocomponente surface comprising a metal or metal oxide; contacting substrate with a first composition selectively associates with the first componentede surface, said first composition comprising a silane, coupling the enzyme to the first composition; yponer contacting the substrate with a second composition selectively associates with segundocomponente surface, said second composition comprising a compound comprising one or masgrupos phosphonic acid or phosphate.