Immunohistochemistry of pituitary gland cells

Immunohistochemistry is a very useful method for identifying the hormone produced in the acinar cells of the pituitary gland. The recent technical progress of the method have let it more popular and easy for many researchers, however, some basic points of the method have become to be missed on the contrary. Immunohistochemical results only show the fact that the immunoreactivity to the antibody remains in the examined tissue, but not the conclusion that the tissue produces hormone A. Some processes are needed in leading the latter conclusion from the former fact. Some necessary points in the process of the immunohistochemistry are discussed in the paper.