Production method of finished-product tissue engineered bone

The invention relates to a production method of a fished-product tissue engineered bone. The method includes: acquiring the mononuclear cell of marrow, and performing multiplication culture to obtain single-cell suspension with the concentration of 2.0*10 -2.0*10 /ml; soaking a porous scaffold into the single-cell suspension to allow the single-cell suspension to enter the porous scaffold sufficiently, and culturing to obtain cell/scaffold composite; inoculating the cell/scaffold composite into a perfusion type bioreactor, adding culture medium and standing for 2-4 hours, and then performing continuous perfusion culture on the cell/scaffold composite to obtain the tissue engineered bone; performing preliminary cleaning, cryopreservation, virus inactivation, ultrasonic cleaning, vacuum freezing drying, aseptic packaging and radiation sterilizing on the tissue engineered bone to obtain the finished-product tissue engineered bone. The method has the advantages that the cryopreservation is performed on the produced tissue engineered bone, and the immunogenicity of the tissue engineered bone can be effectively lowered.